Characterising avenin-like proteins (ALPs) from albumin/globulin fraction of wheat grains by RP-HPLC, SDS-PAGE, and MS/MS peptides sequencing

Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars.

The accurate identification of alleles for high-molecular weight glutenins (HMW-GS) is critical for wheat breeding programs targeting end-use quality. RP-HPLC methods were optimized for separation of HMW-GS, resulting in enhanced resolution of 1By and 1Dx subunits. Statistically significant differences in retention times (RTs) for subunits corresponding to HMW-GS alleles were determined using 1...

Identification of electrophoretic proteins pattern of Fusaium verticillioides isolates from maize by SDS-PAGE.

Study on pattern of Neospora caninumtachyzoite proteins by SDS-PAGE and Western blotting in aborted cows

The intracellular parasite Neospora caninumis prevalent in several countries and is increasingly recognized as an important cause of abortion and stillbirth in cattle. For characterizeing the tachyzoite antigens of Neospora caninumin aborted cows, sera were obtained from 116 cows which were aborted in the third semester of the pregnancy period and had antibodies to Neospora caninumin ELISA. To ...

identification of electrophoretic proteins pattern of fusaium verticillioides isolates from maize by sds-page.

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HPLC of peptides and proteins.

High-performance liquid chromatography (HPLC) is an essential tool for the purification and characterization of biomacromolecules. This unit presents a thorough discussion of the eight types of HPLC currently used, highlighting equipment and start-up procedures, recommendations for running each type of experiment, and theoretical considerations for the separation of peptides and proteins. This ...